DNA is a double helix; a twisted ladder is the description frequently given. The "rungs" of the ladder are made up of pairs of nitrogenous bases: adenine (A), guanine (G), cytosine (C) and thymine (T). The bases will only pair up with one other type each: A with T and G with C. So, the "rungs of the ladder" will each be one of those sets. During replication, when mRNA is formed from one side of the opened-up double helix, the As, instead of pairing with Ts, pair up with a uracil (U). This is because RNA does not utilize thymine, only uracil. The sequence of DNA that you gave:
AACCCTGAGTCT
when transcribed into an mRNA molecule will be:
UUGGGACUCAGA
The information will be carried by the mRNA molecule out of the nucleus of the cell to a ribosome, where it will be translated into a series of amino acids, making a protein.
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